Webinars
04
Out
16:00
Software Tools for NGS-Based Diagnosis of CFTR-Related Diseases in Routine Practice

This webinar will discuss the use of new software tools to support the diagnosis of CTFR-related disorders using next-generation sequencing.

Molecular diagnosis of cystic fibrosis and CFTR-related disorders is based on the detection of mutations in the CFTR gene. A wide range of techniques is still used to identify CFTR gene sequence variations. While there is no gold standard or preferred method for routine testing, the rapid adoption of NGS technologies in diagnostics laboratories is enabling a range of new approaches.

In this webinar, Caroline Raynal of the Laboratory of Molecular Genetics at Arnaud de Villeneuve Hospital will describe how her team tested a new data analysis software in combination with a diagnostic amplicon-based CFTR assay for NGS.

The lab re-analyzed 13 runs in which 158 individuals were included (patients, relatives, partners, and fetuses suspected to have CF) and assessed the assay with the new software.

This webinar will provide details on the findings of this study as well as how amplicon-based solutions for NGS in diagnostics can provide reliable results.

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09
Out
16:00
Analysis of Permanent Gases: More Challenging Than You Might Think
On the surface this would appear to be a simple separation; however, there are several challenges related to this analysis. We will discuss typical techniques utilized for resolving and detecting permanent gases including CO2 and Hydrogen. Other topics will include cryogenic techniques, column isolation, and the use of the “Select Permanent Gas column”.
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09
Out
17:00
Whole Exome Sequencing of Archival FFPE Tissue: A Comparison of Library Prep Methods

This webinar will discuss a comparison of several different library preparation methods for whole-exome sequencing of formalin-fixed paraffin embedded (FFPE) tissue.

FFPE procurement is the standard for tumor banking and remains part of the clinical standard of care. These samples provide an excellent opportunity to advance cancer research with well characterized histological and pathological annotation combined with extensive clinical data.

The ability to use archival FFPE samples to screen entire exomes for both known and novel mutations will have a strong impact on clinical and basic research initiatives, but the use of DNA extracted from FFPE for whole-exome sequencing (WES) is presently limited. The pre-capture PCR step within the WES protocol is the most critical when working with degraded samples, which can therefore significantly affect the quality of sequencing data.

In this webinar, Prashant Singh of the Roswell Park Comprehensive Cancer Center will discuss his team's project to test several different methods for adding adaptors (pre-capture PCR) within the WES protocol. Dr. Singh will share the results of the comparison and the impact of this work on downstream analysis.

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11
Out
17:00
The Bright Side of the Dark Room: My Experience with FISH Applications in Anatomic Pathology
Fluorescence In Situ Hybridization (FISH) is a technology that has slowly invaded the pathology lab over the last two decades, to a point that FISH testing has become an integral part of diagnostic and prognostic/predictive workup in oncologic pathology. This is due to robust, commercially available probes, standardized kits components, and advances in automating the laboratory process and analysis. However, with these advances, the anatomical pathology (AP) lab still faces many challenges when analyzing FISH samples.

This educational webinar will examine the following aspects of implementing FISH:

1. Genetic anomalies detected by FISH
2. The most common FISH assays in an AP laboratory setting
3. Alternative signal patterns that can be observed
4. Pitfalls to avoid during the interpretation process

Participants will gain an understanding of the utility of common FISH assays in the AP lab and a foundation for interpretation of FISH signal patterns.

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19
Out
18:00
Scattered Light Measurements on the Cary 5000 and UMA
Light interaction with materials takes the form of reflectance, transmission, or absorbance. In the case of transmission, the incident radiation can be either transmitted or scattered. This Webinar will focus on the set up and data collection for a scattering sample on the Cary 5000 and UMA. It will also look at the differences in the data collection capabilities between the UMA and an integration sphere.
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25
Out
16:00
Thermal Analysis of Food

In the webinar titled "Thermal Analysis of Food", we describe a number of interesting application examples that demonstrate the use of thermal analysis techniques in fields such as food processing, food storage conditions, and food quality in various segments of the food industry.

A highly regulated industry

Food products are directly linked to public health issues. As a consequence, various international or national regulations and laws exist which provide methods for checking the quality of food products.

For example, in sugar industry, the International Commission for Uniform Methods and Sugar Analysis known as ICUMSA has defined standard methods for the determination of the moisture content in sugar in the production process.

Thermal analysis is nowadays an important technique for characterizing different materials in many fields of the food industry.

Thermal analysis of food

The most important effects that can be analyzed by DSC are the melting point, melting range and melting behavior. DSC is used to determine the heat of fusion, purity, polymorphism, glass transition, and oxidation stability.

The main applications of TGA have to do with evaporation, desorption and vaporization behavior, thermal stability, kinetics of decomposition, and compositional analysis.

TOA is used to study the melting point, melting range, and polymorphism using visual observation and recording images and videos.

TMA is normally used to study the expansion or shrinkage of materials and the glass transition.

DMA is the most sensitive method for characterizing glass transition of materials.

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25
Out
16:00
Automated Data Integrity – Problems and Solutions
This webinar shows how automatic data integrity for the data flow in a quality control lab can be achieved in a lean and efficient way. Improve data quality and sample throughput, use lab technicians time better.
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25
Out
17:00
Introducing SureSelect Cancer All-In-One: Streamlined Detection of All Cancer-relevant Variants in a Single DNA Workflow
Genetic variations underlying cancer consist of SNV, indel, CNV and chromosomal translocations. These diverse types of variations are conventionally analyzed using a range of technologies including FISH, aCGH, PCR, and Sanger sequencing. The recent advances in next generation sequencing (NGS) technologies have enabled unprecedented sensitivity and precision in decoding the genetic landscape of cancer. However, the limitations of currently available methods still preclude the detection of all variant types in a large number of genes at the same time.

Here we introduce the SureSelect Cancer All-In-One solution that enables detection of cancer-relevant SNVs, indels, CNVs and translocations with one streamlined DNA workflow, increasing the efficiency and cost-effectiveness of cancer genetic profiling. SureSelect Cancer All-In-One offers predesigned cancer panels and easy panel customization, a streamlined library prep and target enrichment workflow, and unique software algorithms for advanced CNV and translocation detection.

In this seminar, we will provide an overview of the SureSelect Cancer All-In-One solution and how it helps address key challenges in cancer genetic profiling. We will present data that show the performance of the SureSelect Cancer All-In-One Lung assay, which targets lung cancer-relevant genes for detection of SNVs, indels, CNVs and translocations. Using FISH as the gold standard for copy number and gene rearrangement analysis, our data demonstrate high-level concordance of our method in detecting ERBB2 CNV and ALK translocation in a cohort of clinical cancer samples. We will describe the key components in this solution, including custom panel design using the Agilent SureDesign software, library preparation and target enrichment by SureSelectXT HS, and data analysis and variant reporting by SureCall. With advanced variant detection capability, a streamlined workflow, and panel customization, along with automation options, SureSelect Cancer All-In-One provides a powerful and flexible tool for cancer genetics research.

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