Various types of nucleic acids, such as antisense oligonucleotides, small interfering RNAs (siRNAs), and aptamers, are being explored for their potential in therapeutic applications. The production of synthetic oligonucleotides typically results in numerous impurities due to the inherent inefficiencies in each step of the chemical synthesis process. These impurities include deletion sequences (missing one or more nucleotides) and by-products that often form during the final deprotection or cleavage stages.

Ion pair reversed-phase (IP-RP) chromatography is the dominant technique for the analysis and purification of oligonucleotides.

Attend this webinar to learn how to achieve superior resolution and separation of oligo therapeutics from the many structurally similar impurities found in a crude sample. Understand how to scale up your optimized analytical method to semi-prep and preparative purification with the same column chemistry.

Key Learning Objectives:

• Method development strategies to optimize your oligo separation
• Tips and tricks of how to best scale-up from analytical to semi-prep and preparative purification
• AdvanceBio Oligonucleotide column performance evaluation and competitive comparison