Secondary interactions in HPLC are unintended interactions between the sample and any component of the flow path. They can lead to poor peak shape, poor resolution, and low sample recovery – and make it hard to interpret your chromatogram! An ideal separation is based purely on the primary separation mechanism, whether that is hydrophobicity in reversed-phase or size in size-exclusion chromatography. In reality, secondary interactions can happen in any kind of separation. In this webinar, we will discuss how they happen and how we can mitigate them, with tools such as mobile phase modifiers or inert LC column technology.