PFAS, often referred to as “forever chemicals,” are a diverse class of anthropogenic compounds that have become a major public health concern. Their wide range of structures and frequent presence in laboratory materials create unique analytical challenges. These challenges are especially pronounced for ultra‑short‑chain PFAS, whose high polarity, limited retention on conventional reversed‑phase columns, and tendency to coelute with matrix components make them particularly difficult to separate and quantify. In this webinar, we will discuss key considerations for selecting an LC separation technique and choosing appropriate consumables—along with strategies to address the chromatographic complexities of ultra‑short‑chain PFAS—to help streamline PFAS analysis.